Fig. 7

Schematic of SMART-Exos and their anticancer efficacy. A The design and application of αCD3-αHER2 SMART-Exos as a targeted immunotherapy for breast cancer. B The in vivo antitumor effect of αCD3-αHER2 SMART-Exos. C Representative immunohistofluorescence images of the margin and interior of frozen tumor sections from PBS- and SMART-Exos-treated mice. Blue: nuclei stained with DAPI. Green: CD3 + cells stained with the anti-CD3 antibody. Scale bars: 50 μm. D Quantitative representation of the number of CD3 + cells from each field of view along the margin and interior of each tumor from PBS- and SMART-Exos-treated groups (15 fields of view per region and two mice per group) [38]. Reproduced with permission. Copyright 2020, Elsevier. PDGFR platelet-derived growth factor receptor, TMD transmembrane domain, SMART-Exos synthetic multivalent antibodies retargeted exosome, HER2 human epidermal growth factor receptor 2