Fig. 5

PEDF-sEVs suppress retinal inflammation in OIR mice. A GFAP expression in the retinas of NOR and OIR mice on P12, P14, and P17. B Relative expression of GFAP compared with that of GAPDH (n = 6 mice/group). C PCR analysis of GFAP gene expression in the retinas of NOR and OIR mice on P12, P14, and P17 (n = 6 mice/group). D GFAP expression in the retinas of OIR mice treated with PBS, PEDF, sEVs, and PEDF-sEVs treated on P17. E Relative expression of GFAP compared with that of GAPDH (n = 9 mice/group). F PCR analysis of GFAP gene expression in the retinas of OIR mice treated with PBS, PEDF, sEVs, and PEDF-sEVs on P17 (n = 6 mice/group). G GFAP expression in the retinas of OIR mice treated with PBS, anti-VEGF drugs, and PEDF-sEVs on P17. H Relative expression of GFAP compared with that of GAPDH (n = 6 mice/group). I Representative immunofluorescence staining images of retinas showing GFAP expression in NOR and OIR mice treated with PBS, PEDF, sEVs, anti-VEGF drugs, and PEDF-sEVs on P17; scale bar = 50 μm. J The fluorescence intensity was measured by integrated density using the ImageJ software (n = 3 mice/group, 3 sections per mouse, at least 3 images per section were analysed and the values were averaged). K, L PCR analysis of TNF-α and IL-1β gene expression in the retinas of OIR mice treated with PBS, PEDF, sEVs, and PEDF-sEVs on P17 (n = 6 mice/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. RGC: Retinal ganglion cell; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; RPE: retinal pigment epithelium