Fig. 6
PEDF-sEVs are superior to anti-VEGF drugs in protecting retinal structure and in ameliorating retinal function in OIR mice. A Representative OCT images of NOR and OIR mice treated with PBS, PEDF, sEVs, anti-VEGF drugs, and PEDF-sEVs on P25 and P42. Red arrows indicate the total retina and green arrows indicate photoreceptor layer. B Quantitative analysis of total retina and photoreceptor layer thickness on P25 (n = 8–10 mice/group). C Quantitative analysis of total retina and photoreceptor layer thickness on P42 (n = 6–7 mice/group). D H&E staining of retinal tissues in NOR and OIR mice treated with PBS, PEDF, sEVs, anti-VEGF drugs, and PEDF-sEVs on P25 and P42 (n = 3 mice/group); scale bar = 50 μm. E Representative TUNEL assay images of eye sections of retinas from OIR mice treated with PBS, PEDF, sEVs, anti-VEGF drugs, and PEDF-sEVs on P17; scale bar = 50 μm. F Quantitative analysis of TUNEL-positive cells in the retinas (n = 5 mice/group, 5 sections per mouse, at least 5 images per section were analysed and the values were averaged). G Representative images of ERG response at a flash strength of 1.0 log (cd•s/ m2) in NOR and OIR mice treated with PBS, PEDF, sEVs, anti-VEGF drugs, and PEDF-sEVs on P25 and P42. H ERG analysis of a and b wave amplitudes on P25 (n = 7–11 mice/group). I ERG analysis of a and b wave amplitudes on P42 (n = 7–9 mice/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. RGC: Retinal ganglion cell; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; RPE: retinal pigment epithelium