Fig. 5

SZP mediates ACD. A The dose–response curve for CCK8 assay of IC50 of STF62247 in different concentrations treating cells at 37 °C and 47 °C for 24 h (n = 3). B The dose–response curve for CCK8 assay of IC50 of STF62247 and SZP NPs in different concentrations treating HCC cells at 47 °C for 24 h (n = 3). C, F Live/dead cells double staining and quantitative analysis of sublethally heated SMMC7721 and Huh7 cells exposed to different groups (n = 4). Scale: 1000 µm. D Analysis of the proliferation of sublethally heated SMMC7721 and Huh7 cells under influence of different groups by CCK8 (n = 3). E, G Representative flow cytometry plots and quantitative analysis of apoptosis in sublethally heated SMMC7721 and Huh7 cells under influence of different conditions (n = 3). H Immunoblotting of p-mTOR, t-mTOR, P62, and LC3B in sublethally heated SMMC7721 and Huh7 cells under influence of different groups. The results were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. ACD, Autophagy-dependent cell death; IC50, Half maximal inhibitory concentration