Fig. 7

Autophagy-dependent ICD induced by SZP and immune vaccine experiments in vivo. A Flow chart of in vivo detection of ICD in tumor-bearing mice. B, D Detection and quantitative analysis of mature DCs (of CD11C⁺ gate) in tumor-draining lymph nodes via flow cytometry (n = 3). C, E–G Expression and quantitative analysis of CRT, HMGB1, and LC3B in tumors via CLSM (n = 3). H Flow chart of in vivo inoculation with immune vaccine. I: Kaplan–Meier plot of survival rate in different groups of tumor-free mice (n = 10). J, K Comparison of tumor growth curves and tumor weights in mice of different groups (n = 6). L Representative IHC images showing the expression of CD4 and CD8 in mice tumor of different groups. M, N Representative flow cytometry plots and quantitative analysis of TEM (of CD8⁺ gate) in the spleen of mice (n = 3). O The levels of IFN-γ and TNF-α in mice serum from inoculated with different immune vaccines by ELISA (n = 4). Results were expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. IHC, Immunohistochemistry; TEM, effector memory T cells