Fig. 2
UBR4 concentration was significantly reduced in EXOUBR4− but exosome characteristics remained unchanged. (A) Transfection of ADV-Sh-UBR4 into MenSCs; (B) Detection of UBR4 mRNA levels by RT-PCR; (C) Western blot of UBR4 of MenSCs; (D) Western blot of UBR4, CD81, and TSG101 of EXOUBR4− and EXO; (E) Size distribution of EXOUBR4− and EXO examined by NTA; (F) Morphology of MenSCs-EXO observed by TEM (scale bar = 1 μm). Data were mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 for Student’s t-test