Fig. 5
UBR4 inhibited endometrial fibrosis through ubiquitinated degradation of YAP. The IUA model rats and IUA-EndoSCs were treated with EXO and EXOUBR4−, respectively. (A) Immunofluorescence assays of YAP (red) and DAPI was used to stain nuclei (Blue) in IUA-EndoSCs (upper) (Scale bar = 50 μm)and endometrium of IUA rats (lower). (Scale bar = 20 μm); (B) Western blot (left) and relative quantification (right) of YAP in the endometrium of IUA rats (upper) and IUA-EndoSCs (lower); (C) CO-IP assay of UBR4 and YAP were performed to investigate the interactions between UBR4 and YAP in IUA-EndoSCs; (D) Immunoprecipitation to determine YAP ubiquitination in IUA-EndoSCs before and after treatment. Data were mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 for One Way ANOVA