Fig. 6

P65 transcriptional activation promoted UBR4 expression in MenSCs. (A) Transfection of ADV-OV-P65 into MenSCs; (B) Detection of P65 mRNA levels by RT-PCR; (C) Western blot of P65 in MenSCs; (D) Transfection of ADV-Sh-P65 into MenSCs; (E) Detection of P65 mRNA levels by RT-PCR; (F) Western blot of P65 in MenSCs; (G) Immunofluorescence assays of P65 (red) and DAPI was used to stain nuclei (Blue) in MenSCs; (H) Western blot (left) and relative quantification (right) of UBR4 in MenSCs with or without overexpression of P65; (I) Western blot (left) and relative quantification (right) of UBR4 in MenSCs with or without knockdown of P65; (J) ChIP to evaluate P65 enrichment in the promoter region of UBR4 gene; (K) Dual-luciferase assay was performed to detect the activity and showed that UBR4 bond PEX3‐P65. Data were mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 for One Way ANOVA