Fig. 5

Effects of VEGF–aPMNEM–ECM on wound healing. (A) Digital images of wounds treated with PBS, ECM, aPMNEM–ECM, and VEGF–aPMNEM–ECM on days 1, 3, 5, 7, and 14. (B) Quantitative analysis of relative wound area rates on days 1, 3, 5, 7, and 14. (C) H&E staining and Masson staining of the wound section. The area marked by the red line in the middle reflected the wound closure. (D) VEGF–aPMNEM–ECM contributed to angiogenesis in vivo. Quantitative analysis of CD 31 (E) and α-SMA (F) positive area. Data are represented as the mean ± standard deviation (n = 5). Analysis of variance was performed; “*”, “#” and “&”: p < 0.05; “**”, “##” : p < 0.01; “***” : p < 0.001. “*” stands for comparison with the PBS group, “#” stands for comparison with the ECM group, “&” stands for comparison with the aPMNEM–ECM group