Fig. 3

dNPc-exo induces M1 polarization of macrophages via miR-27a-3p. (A) Representative images of immunofluorescence staining of Cy3-labelled miR-27a-3p in M0 macrophages. Scale bar = 10 μm. (B) Representative images of immunofluorescence staining of macrophages after cultured with dNPc-exo, miR-27a-3p mimic and miR-27a-3p inhibitor (blue, nucleus; green, F4/80; red, iNOS). Scale bar = 100 μm. (C) Quantitative analysis of the iNOS⁺ macrophages percentage in macrophages after cultured with dNPc-exo, miR-27a-3p mimic and miR-27a-3p inhibitor. (D) RT-qPCR analysis of the miR-27a-3p expression in macrophages of control, nNPc-exo and dNPc-exo group. (E) Western blot analysis of iNOS expression in M0 macrophages after treatment of dNPc-exo, miR-27a-3p mimic and miR-27a-3p inhibitor. (F) Quantitative analysis of iNOS expression in western blot of M0 macrophages after treatment of dNPc-exo, miR-27a-3p mimic and miR-27a-3p inhibitor. The data are expressed as the mean ± SEM. n = 3. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non-significant difference