Fig. 5

Comprehensive verification of the molecular mechanism of the PPARγ/NFκB/PI3K/AKT regulatory axis of dNPc-exo-carried miR-27a-3p. (A) RT-qPCR analysis of PPARγ, NFκB, PI3K, AKT and iNOS gene expression in M0 macrophages after cultured with dNPc-exo. (B) RT-qPCR analysis of PPARγ, NFκB, PI3K, AKT, iNOS and miR-27a-3p gene expression in M0 macrophages after cultured with dNPc-exo mimic and inhibitor. (C) Western blot analysis of PPARγ, NFκB, PI3K, AKT, iNOS and and TNF-α expression in M0 macrophages after cultured with nNPc-exo and dNPc-exo. (D) Quantitative analysis of PPARγ, NFκB, PI3K, AKT, iNOS and TNF-α expression in western blot after cultured with nNPc-exo and dNPc-exo. The data are expressed as the mean ± SEM. n = 3. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, non-significant difference