Fig. 2

circ_0001785 protection in populations. A qRT-PCR analysis of circ_0001785 and linear ELP3 mRNA expression after RNase R digestion. B Sanger sequencing verification. C circ_0001785 and GAPDH expression in endothelial cell cDNA and gDNA (using divergent primers and polymerization primers, respectively). D The sequence of has_circ_0001785. E circ_0001785 had stable expression in monocytes. F Expression of circ_0001785 by qRT-PCR assay on CHD or non-CHD. G Expression of circ_0001785 in plaque tissue of patients with lower extremity atherosclerotic plaques. H Expression levels of circ_0001785 in patients with severe versus moderate coronary artery stenosis. Results are expressed as the mean ± SEM; student’s t-test or one-way ANOVA. *P < 0.05, **P < 0.01, **** P < 0.0001