Fig. 4

circ_0001785 exerts its functional through miR-513a-5p/TGFBR3ceRNA network axis. A Relative luciferase reporter activity of vectors carrying the luciferase gene and a fragment of circ_0001785 wild-type or mutant-type of miR-513a-5p binding sites after cotransfection with mimic NC or mimic miR-513a-5p. B The possible binding sites between circ_0001785 and miR-513a-5p. C Relative luciferase reporter activity of vectors carrying the luciferase gene and a fragment of TGFBR3 wild-type or mutant-type of miR-513a-5p binding sites after cotransfection with mimic NC or mimic miR-513a-5p. D The possible binding sites between TGFBR3 and miR-513a-5p. E, F qRT-PCR assay for the detection of miR-513a-5p and TGFBR3 expression in the blood of coronary heart disease and healthy patients. G, H qRT-PCR assay for the detection of miR-513a-5p and TGFBR3 expression in the plaque and paraplegic. Results are expressed as the mean ± SEM; student’s t-test or one-way ANOVA. *P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001