Fig. 5

In vitro cytotoxicity of B7H3/DOX@GNCs in NCI-H1299 cells. A, B Cytotoxicity of B7H3/DOX@GNCs under with diffrent NIR irradiation time on NCI-H1299 cells for 24 h. C Cytotoxicity of different concentrations of GNCs, Dox, DOX@GNCs, B7H3@GNCs, and B7H3/DOX@GNCs with/without irradiation on NCI-H1299 cells for 24 h and cell viability was assessed by MTT assay. D Live/dead cell staining assay results of NCI-H1299 cells treated with GNCs, B7H3@GNCs, DOX@GNCs, and B7H3/DOX@GNCs with or without NIR radiation. Live cells were stained with calcein (green), and dead cells were stained with propidium iodide (red). Data are Mean ± SD (n = 3), ^*P < 0.05 and ^**P < 0.01, (one-sample t-test) versus GNCs group