Fig. 4
(Reproduced under the terms of the CC-BY 4.0. Copyright 2019, The Authors, published by BioMed Central on behalf of the Journal of Nanobiotechnology)
Targeted delivery of miR-210 by chemically modified EVs for MCAO treatment [145]. A Schematic diagram of RGD modification on EV surface by EDC/NHS coupling chemistry and click chemistry. B NIRF imaging of the MCAO mouse brains 6 h after the intravenous injection of PBS, unmodified EVs with miR-210 (Exo:miR-210), scramble peptide-modified EVs with miR-210 (Scr-exo:miR-210), and RGDyK-modified EVs with miR-210 (RGD-exo:miR-210) (EVs labeled with Cy5.5). C The mRNA level of VEGF in the lesion region of MCAO mice 24 h after the intravenous injection of PBS, Scr-exo:miR-210, RGD modified EVs (RGD-exo), RGD modified EVs with controlled RNA (RGD-exo:NC), and RGD-exo:miR-210 (**P < 0.01 vs the Sham group; #P < 0.05, ##P < 0.01 vs RGD-exo:miR-210 group using one-way ANOVA followed by Tukey’s post hoc test). D Survival rate of MCAO mice after the intravenous administration of RGD-exo-NC and RGD-exo:miR-210