Fig. 2
In vitro evaluation of the effects of zinc (Zn2+)-mediated osteoclastogenesis. a Cell counting kit-8 assay of the survival of bone marrow monocytes (BMMs). b qPCR analysis of osteoclast gene expression in BMMs. c Trap staining of osteoclasts induced by different doses in a Zn2+-enriched environment. d, e Immunofluorescence staining of actin ring formation and statistical analysis (n = 3, *p < 0.05, **p < 0.01)