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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Dominoes with interlocking consequences triggered by zinc: involvement of microelement-stimulated MSC-derived exosomes in senile osteogenesis and osteoclast dialogue

Fig. 5

MicroRNAs (miRNAs) identified in aged extracellular vesicles. a, b Venn diagram and volcano plot analysis of the total miRNAs from aged exosomes with zinc (AZ-EXOs) and without zinc (A-EXOs). c Heatmap of differentially expressed miRNAs between AZ-EXOs and A-EXOs. d, e Bubble plot showing Gene Ontology enrichment in molecular function and signaling pathway enrichment in Kyoto Encyclopedia of Genes and Genomes pathways. f Network plot of the interactions of enriched biological pathways. g High expression of miRNA-450a-3p, miRNA-301b-3p, miRNA-223-5p, and miRNA-6328 in AZ-EXOs was confirmed using RT-PCR. h, i Dual-luciferase assay was conducted for wild-type and mutated miR-146b-5p recognition sites within the PI3CK 3’UTR. j Western blot and quantitative analysis of PI3CK protein levels (n = 3, *p < 0.05, **p < 0.01)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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