Fig. 6

miR-146b-5p silencing attenuated the effect of aged exosomes with zinc (AZ-EXOs) in bone marrow monocytes (BMMs). a BMMs were treated with miRNA-146b-5p-inhibitor for 6 h and the expression was tested by RT-PCR. b Cell counting kit-8 detection of BMMs with or without inhibitors. c RNA expression levels of CTR/TRAP/CTSK in BMMs. d TRAP staining of osteoclasts. e Representative scanning electron microscopy images of the bone resorption pits. d Western blot analysis of the phosphatase and tensin homolog (PTEN)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway and its phosphorylation activation.