Fig. 5
miR-199a-3p transferred by MSCsSC-Exos decrease the expression of mTOR in OA model. A Heatmap showing the hierarchical cluster of differential miRNA expression in MSCsSC-Exos. B The relative expression of miR-199a-3p on OA cartilage with MSCsSC-Exos treatment was detected by RT-PCR. C The relative expression of miR-199a-3p on chondrocytes with MSCsSC-Exos treatment was detected by qRT-PCR. D The effects of MSCsSC-Exos and antagomir-199a-3p on the luciferase activity of the IL-1β-induced chondrocytes. E Schematic representation of a predicted binding site of miR-199a-3p in the 3ʹUTR of mTOR mRNA, and the mutant mTOR 3ʹUTR. F The luciferase activity was determined using the dual-luciferase reporter system. G Protein levels of mTOR and P-p70s6 were examined by western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001, ns not significant