Table 1 The primer sets for qRT-PCR were listed below
Gene | Forward primer (5ʹ-3ʹ) | Reverse primer (5ʹ-3ʹ) |
|---|---|---|
rno ACTIN | AAGGCCAACCGTGAAAAGAT | TGGTACGACCAGAGGCATA |
rno MAP1LC3B | TGTTAAGCCCCTACCAAGGC | GCATGGCACTCAGTTTCTGG |
ATG9A | CGAGGCTGGTAACTGGAATC | CCCAGCCCTTAGCACTTAGAC |
Atg4C | GTAGTCCGAGACCGCAGGAG | GAGCCTAGCGCCGTGTATTT |
TGFB1 | GACTCTCCACCTGCAAGACC | GGACTGGCGAGCCTTAGTTT |
WIPI2 | GCATGGAGACGACCAGTGAA | TTGGCGTCCTGTCCTTTACC |
HTT | TGCCCGTGTAAAGTGTGTGA | AAGTGCCATAGGGTGTTGGG |
PIK3C3 | AGATGGTCGAAGGGATGGGT | AGGGCGTGAACACTTTCGTC |
AMBRA1 | GGCGTTCGTGTGTTCGATG | CTCATGGAAATCTGCGAGGC |
TP53 | CCCCTGAAGACTGGATAACTGT | CAGGAGCTGACACTTGGAGG |
BCL2L1 | AGATTCGGCACGAGCAGTC | TGGGCTCAACCAGTCCATTG |
BNIP3 | TTGGGTGGGCAGTTGTGTTA | TACAGCATGTCAAGGAGGCA |
MAPK8 | CTCTCCAGCACCCGTACATC | TTGACAGACGGCGAAGAGAC |
CLN3 | CGCTAACTCCTAACACACGC | GTTTCCAGATGCCTGCTCCT |
VTILA | CTCAGAGAACCAGAGGGCAC | CCCAGTCAGAATCCGAGAGC |
ATP6V0A1 | GAGGACGCAGAAGAGCCTAC | AGGACGCAGTGTTGGAGATG |
IGF1 | CAAAATGAGCGCACCTCCAA | CTTCAGCGGAGCACAGTACA |
HDAC6 | GCTCAGGAATCTTGGGAGCA | AAGAATCTTGGCCGGTGGAG |
BAX | CACGTCTGCGGGGAGTCAC | TTCTTGGTGGATGCGTCCTG |
BAK1 | GTTTCTCGGGCTAAAAGGCG | CCAGATGTGGAATGGCCCAG |
MAPK14 | GGATATTTGGTCCGTGGGCT | TCATGGCTTGGCATCCTGTTA |
DAPK1 | GGTTCCCACTCACTCCTTCTG | CCAAGTGACCGAAGTGAACC |
SLC7A5 | GCGTGAAAACTGCTGGCTAC | TCACCCCAACACAAGGGAAC |
mTOR | CTGCACTTGTTGTTGCCTCC | TGGTCTAGCGTGCGAACAAT |
hsa U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
Rno/mmu U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCAT |
rno/has/mmu miR-199a-3p | CGGCGGACAGTAGTCTGCACA | CAGTGCAGGGTCCGAGGTAT |
rno/has/mmu miR-199a-3p stem loop primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCA | |