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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Exosomes derived from LPS-preconditioned bone marrow-derived MSC modulate macrophage plasticity to promote allograft survival via the NF-κB/NLRP3 signaling pathway

Fig. 1

Identification of Exo. A TEM images of Exo and LPS pre-Exo. B The diameter of exosomes were detected by NTA analysis (n = 3). C The enhanced secretion of exosomes detected by BCA assay after LPS pretreatment (n = 3). Data are presented as mean ± SD.*P < 0.05. D The markers (HSP90B1, CD63, TSG101, CD9) of exosomes analyzed by Western blot. (Full-length blots are presented in Additional file 1: Fig. S2). E Macrophage phagocytosis of Exo and LPS Pre-Exo was observed under a fluorescence microscope. F, G Representative image of exosome (PKH26+) Uptake by Macrophages (F4/80.+) with flow cytometry analysis. Values are presented as the mean ± SD (n = 3). *P < 0.05

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