Fig. 2

Effects of LPS pre-Exo on the survival of allogeneic skin grafts in vivo. A Schematic diagram of allogeneic skin grafting. The allografts Skin from BALB/c were transplanted into C57 recipient mice. B, C The necrosis of allograft skins was observed immediately after operation and at postoperative day 7, 14, 21, 23. Necrosis was observed at post-transplanted day 7 in the control group. The survival time of transplanted skins was prolonged by both rapamycin (1 mg/kg) and Exo (50 mg/kg), while LPS pre-Exo(50 mg/kg) was more effective (n = 5). B The percentage of allograft skin necrosis after transplantation (n = 5). D, E Representative H&E staining images of skin graft (day 7) in various groups. E Quantification of allograft rejection score in transplanted skin tissue in each group (n = 7). *P < 0.05, ****P < 0.0001