Fig. 3

Effects of LPS pre-Exo on the polarization of macrophages in transplanted skin. The allogeneic skins were harvested at postoperative day 7. A Representative images of immunohistochemical staining of F4/80, iNOS and CD206 of transplanted skin in various groups. B Representative immunofluorescence images of F4/80 and iNOS in the transplanted skin tissues were obtained from each group of recipient mice. C Representative images of immunofluorescence of F4/80 and CD206 in allografted skin tissues of recipient mice in each group. D, E The data of positive cells in immunofluorescence images are shown as mean ± SD with One-way ANOVA analyses (n = 5). ns > 0.05, * P < 0.05, ** P < 0.005. F4/80: green; iNOS: red;CD206: magenta; DAPI: blue. Scale bars: 50 μm