Fig. 4

Effects of LPS pre-Exo on macrophage polarization in allograft recipients. A Gating strategy for detection of spleen macrophages by flow cytometry. B, C Representative images of CD86⁺and CD206⁺ at postoperative day 7 in various groups of spleen macrophages analyzed by flow cytometry. Values are presented as the mean ± SD (n = 3). ns > 0.05, *P < 0.05, **P < 0.005, **** P < 0.0001. D, E Representative images of CD86⁺and CD206.⁺ at postoperative day 14 in various groups of spleen macrophages analyzed by flow cytometry. Values are presented as the mean ± SD (n = 3). ns > 0.05, *P < 0.05, **P < 0.005, **** P < 0.0001