Fig. 6

Effects of LPS pre-Exo on protein expression levels in macrophages. A The concentrations of cytokine (IL-2, IL-6, IL-1β, IL-18) were detected by Elisa in the macrophage supernatant of each group.(n = 4). B, C BMDM cells were pretreated with LPS (100 ng/m) for 3 h before culturing with LPS pre-Exo for 24 h. Protein expressions of NF-κB, NLRP3, Procaspase-1 and ASC in macrophages of each group analyzed by western blot. (Full-length blots are presented in Additional file 1: Fig. S3) The effect of LPS pre-Exo on protein expression in the macrophages compared with bm-Exo (n = 3). D, E BMDM cells were pretreated with LPS (100 ng/m) for 3 h before culturing with LPS pre-Exo for 48 h. Protein expression of NF-κB, NLRP3, Procaspase-1 and ASC in the macrophages of each group analyzed by Western blot. (Full-length blots are presented in Additional file 1: Fig. S4) The effect of LPS pre-Exo on protein expression in the macrophages compared with bm-Exo. (n = 3). A, C, E Data are presented as mean ± SD. *P < 0.05, **P < 0.005, *** P < 0.0005, **** P < 0.0001