Fig. 2
The infiltration of Treg cells in the spinal cord can help the recovery of TJs protein expression and motor function in mice with spinal cord injury. A, B Co-immunostaining and quantification of TJs protein and blood vessels at the injured site on day 7 after spinal cord injury (arrows: intact blood vessels; triangles: damaged blood vessels) (n = 3). C The expression of TJs protein in the spinal cord of the sham group, PBS group, Treg cell knockout group, and transplantation group was detected by western blotting. D, E Footprint analysis showed the recovery of motor function at 28 days after spinal cord injury in Treg cells knocked out and transplanted mice (n = 6). F Swimming tests showed recovery of motor function 28 days after spinal cord injury in Treg cells knocked out and transplanted mice. G Louisville Swim Scale in Treg cells knocked out and transplanted in mice (n = 6). H BMS scores showed the recovery of motor function at 28 days after spinal cord injury in Treg cells knocked out and transplanted in mice (n = 6)