Fig. 3

Treg-Exos promote the expression of TJs protein in vitro. A, B Immunofluorescence staining and quantization of TJs protein in the OGD/R group, the OGD/R + T-CM group, and the GW4869 group (n = 3). C The expression of TJs protein in three groups of bEND.3 cells were detected by western blotting. D TJs integrity of bEND.3 cells in each group were evaluated by FITC-Dextran (n = 3). E TJs integrity of bEND.3 cells in each group were evaluated by TEER (n = 3). F–H TEM, NTA analysis, and Western blot were used to identify exosomes