Fig. 4

Treg-Exos promote the expression of TJs protein in vivo and improve the exercise ability of mice. A, B Co-immunostaining and quantification of TJs protein and blood vessels in the spinal cord of mice on day 7 after spinal cord injury (arrows: intact blood vessels; triangles: damaged blood vessels) (n = 3). C, D The fluorescence intensity and quantification of FITC-dextran in the spinal cord at day 7 after spinal cord injury were used to detect the permeability of BSCB (n = 4). E Western blot was used to detect the expression of TJs protein in the spinal cord after Treg-Exos intervention. F–K Footprint analysis (n = 6), MEP analysis (n = 3), and BMS scores (n = 6) were used to evaluate the locomotor ability at day 28 after Treg-Exos intervention in spinal cord injury mice