Fig. 5

Treg-Exos promote TJs protein expression in vitro by delivering miR-2861. A The transfection efficiency of miR-2861 in Treg cells was verified by qRT-PCR. B The relative expression of miR-2861 in Treg-Exos. C The relative expression of miR-2861 in miR-NC^KD-Exos, miR-2861^KD-Exos, miR-NC^OE-Exos, and miR-2861^OE-Exos-treated bEND.3 cells. D, E Expression and quantification of TJs protein in miR-NC^KD-Exos, miR-2861^KD-Exos, miR-NC^OE-Exos, and miR-2861^OE-Exos-treated bEND.3 cells. F Western blotting was used to detect the expression of TJs protein in miR-NC^KD-Exos, miR-2861^KD-Exos, miR-NC^OE-Exos, and miR-2861^OE-Exos-treated bEND.3 cells. G, H FITC-dextran and TEER values were used to assess the TJs permeability of miR-NC^KD-Exos, miR-2861^KD-Exos, miR-NC^OE-Exos, and miR-2861^OE-Exos-treated bEND.3 cells (n = 3)