Fig. 7

miR-2861 promotes BSCB repair by inhibiting IRAK1 expression. A miR-2861 in Treg-Exos can bind to the 3'-UTR to inhibit IRAK1 expression. B A luciferase report assay was used to verify IRAK1 as a downstream target gene of miR-2861 (n = 3). C qRT-PCR and western blot were used to verify that miR-2861 could negatively regulate the relative expression of IRAK1 (n = 3). E, F Expression and quantification of TJs protein in Treg-Exos-treated bEND.3 cells. G, H FITC-dextran and TEER values were used to assess the TJs permeability of Treg-Exos-treated bEND.3 cells (n = 3). I, J Expression and quantification of TJs protein in Treg-Exos-treated bEND.3 cells. K, L FITC-dextran and TEER values were used to assess the TJs permeability of Treg-Exos-treated bEND.3 cells (n = 3)