Fig. 3
From: Cell-surface photochemistry mediated calcium overload for synergistic tumor therapy
Analysis of antitumor mechanism in vitro. A Fluorescence images of the distribution of CD20 receptors on Raji cell surface with or without NIR irradiation. B Confocal microscopy images of ROS generation in Raji cells after various treatments. C Evaluation of intracellular ROS production using the DCFH-DA probe in Raji cells after treatment with different preparations via flow cytometry. D Intracellular ROS generation was detected in Raji cells treated with photonanomedicine under 980 nm NIR irradiation for various time periods. E Confocal microscopy images of Raji cells stained with Fluo-4 AM after different treatments. F The flow cytometry analysis of intracellular Ca2+ in Raji cells after various treatments. G The change of mitochondria membrane potential of Raji cells after different treatments was objectively measured using flow cytometry. H Expression of anti-apoptotic proteins (Bcl-2 and Bcl-xL) and pro-apoptotic proteins (Bak, Bax and Cleaved Caspase-3) of Raji cells after various treatments. I, J Immunoblot analysis of Cyt c expression in mitochondria and cytoplasm of Raji cells after various treatments. (K) Schematic diagram of the signal events involved in the apoptosis process. 1#: Control; 2#: NIR; 3#: Photonanomedicine; 4#: PpIX-lacking photnanomedicine + NIR; 5#: CA-lacking photonanomedicine + NIR; 6#: Photonanomedicine + NIR.