Fig. 4

A TEM images of sEV extracted from the model cell line GES-1 (upper scale: 100 nm, lower scale: 50 nm). B NTA analysis of sEV from GES-1. C Western blot analysis of sEV marker proteins CD-81, CD-9 and CD-63. D DPV signal of extracted sEV from three model cell lines GES-1, MKN-28 and AGS. E RT-qPCR analysis of extracted sEV from three model cell lines GES-1, MKN-28 and AGS. The error bars are the standard deviations by three repetitive assays (n = 3)