Skip to main content
Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Designing a microbial fermentation-functionalized alginate microsphere for targeted release of 5-ASA using nano dietary fiber carrier for inflammatory bowel disease treatment

Fig. 4

Inflammatory activity analysis of colonic tissues. A and B Representative confocal images of neutrophils (CD11b+ Ly6G+) and macrophages (CD11b+ F4/80+) among all treatment groups. C and D Flow cytometry dot plots and quantitative analysis of both cell populations. Single-cell suspensions were derived from colonic tissues of mice with colitis after 30 days of different drug treatments. Each single-cell suspension from different treatment groups was divided into two equal parts. One was stained with Hoechst 33,342 (blue), FITC-labeled CD11b antibodies (green) and PE-labeled Ly6G antibodies (red) to visualize the nuclei and neutrophils, and the other was stained with Hoechst 33,342 (blue), FITC-labeled CD11b antibodies (green) and PE-labeled F4/80 antibodies (red) to visualize the nuclei and macrophages. The merge of the three colors represents the number of cells. The number of both cell populations was further quantified by flow cytometric analysis. *p < 0.05 and ***p < 0.001 versus the model group. All data are presented as the mean ± SD (n = 6)

Back to article page

Journal of Nanobiotechnology

ISSN: 1477-3155

Contact us