Fig. 5

Hypo-Exo protected vascular endothelial cells from I/R damage in vivo and vitro. A CD31 immunohistochemical detection in each group (Original magnification 40X, bar size 20 μm). B Representative images showing tube formation in HUVECs treated with PBS, OGD + PBS, OGD + Hypo-Exo or OGD + Exo (bar size 200 μm). C Representative images showing migrated HUVECs by transwell assay treated with PBS, OGD + PBS, OGD + Hypo-Exo or OGD + Exo (bar size 50 μm). D The number of CD31 positive blood vessels in each group (**P < 0.01, *P < 0.05). E Quantitative data of tube formation using Image J (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). F Quantitative data of the migrated cells (*P < 0.05, ***P < 0.001, ****P < 0.0001) G, H Western blot shows the expression of VEGF-A in flap treated with each group, and quantitative data of western blot (*P < 0.05, **P < 0.01, ***P < 0.001), Bars indicated means ± SD