Fig. 7
3-MA inhibit autophagy reversed the biological effects promoted by Hypo-Exo in vivo and vitro. A Representative skin flap appearance of each group on the7th day after operation. B Representative histology images of each group on the 7th day after operation C CD31 immunohistochemical detection in each group (Original magnification 40X, bar size 20 μm) D Quantitative data of CD31 positive blood vessels in each group (**P < 0.01) E Double immunofluorescence staining of Dihydroethidium (DHE)-ROS (red) and DAPI (bule) in each group. F Statistical evaluation of Mean fluorescence intensities in each group (**P < 0.01). G Double immunofluorescence staining of TUNEL (green) and DAPI (bule) in each group. H Quantitative data of apoptotic cells and TUNEL cells (**P < 0.01). I, J Western blot analysis of the expression of proteins in flap, and quantitative data of western blot (*P < 0.05). K Representative images showing tube formation in HUVECs treated with OGD + Hypo-Exo, OGD + Hypo-Exo + 3-MA (bar size 200 μm). L Representative images showing migrated HUVECs by transwell assay treated with OGD + Hypo-Exo, OGD + Hypo-Exo + 3-MA (bar size 50 μm). M Quantitative data of tube formation using ImageJ (*P < 0.05, ** P < 0.01). N Quantitative data of the migrated cells (**P < 0.01), Bars indicated means ± SD