Fig. 3

The in vitro interaction of LDC@ZnP NPs and BMDCs. A Confocal images of BMDCs after incubation with L@ZnP NPs and LDC@ZnP NPs. Cell nuclei were stained with DAPI, scale bar = 50 μm. B Quantitative uptake of L@ZnP NPs and LDC@ZnP NPs by BMDCs detected by flow cytometer (n = 3), ns: not significant, ***p < 0.001, ****p < 0.0001. C The OD values of BMDCs incubated with different amounts of LDC@ZnP-MA NPs (n = 4). D Flow scatter plots of CD11c⁺CD86⁺ cells. E Quantitative analysis of CD11c⁺CD86⁺ cells (n = 3), *p < 0.05. F The level of IL-6 in the supernatant of BMDCs (n = 3), *p < 0.05. G The level of TNF-α in the supernatant of BMDCs (n = 3), *p < 0.05