Fig. 7

The antitumor immune responses elicited by LDC@ZnP NPs. The mice were treated with PBS, PBS + NIR, Free M + A, Free M + A + NIR, LDC@ZnP-M NPs + NIR, LDC@ZnP-A NPs and LDC@ZnP-MA NPs + NIR, respectively. A Flow scatter plots of CD11C⁺CD86⁺ cells in TDLNs. B Flow scatter plots of CD3⁺CD8⁺ cells in TDLNs. C Flow scatter plots of CD3⁺CD8⁺ cells in spleens. D Flow scatter plots of CD3⁺CD8⁺ cells in tumor tissues. E Quantitative analysis of CD11C⁺CD86⁺ cells in TDLNs (n = 4), *p < 0.05. F Quantitative analysis of CD3⁺CD8⁺ cells in TDLNs (n = 4), *p < 0.05. G Quantitative analysis of CD3⁺CD8⁺ cells in spleens (n = 4), *p < 0.05. H Quantitative analysis of CD3⁺CD8⁺ cells in tumors (n = 4), *p < 0.05. I Serum levels of TNF-α (n = 3), * p < 0.05. J Serum levels of IL-6 (n = 3), *p < 0.05. K Immunofluorescent staining of IL-6 in tumor sections. The nuclei were stained with DAPI, scale bar = 200 μm