Fig. 4

miR-5590 regulates DDX5 and mTOR expression. (a) Analysis of DDX5 and miR5590 binding sites. (b) The volcano plot of differential expression of miRNAs in IVDD. Red circles denote miR-5590 under-expressed in degenerative intervertebral disc. (c) Real-time PCR detection of gene expression in NPCs. (d) Western blotting data showing the levels of the expression of DDX5, p-mTOR, and mTOR. (e, f) Western blotting data of the levels of the protein in (d). Data are expressed as average ± SD (n = 3). **P < 0.01; ***P < 0.001