Fig. 2
A The average size distribution and (B) zeta potential of HBsAg, HPLNP and HBsAg/HPLNP (w/w = 1/600) formulation detected by DLS. C The TEM graph of the HPLNP. D Cumulative release of BSA from different formulations including BSA, BSA/Al and BSA/HPLNP (w/w = 1/600) in PBS at pH 7.4 for 72 h. E The cell viability of RAW264.7 cells after being treated with HBsAg, HBsAg/Al or HBsAg/HPLNP (w/w = 1/600) formulation at different concentrations of HBsAg for 24 h. F The haemolysis of alum adjuvant and HPLNPs at the same concentration of 0.5 mg mL−1. *p < 0.05, **p < 0.01, ****p < 0.0001. Student’s t-test, Two-way ANOVA test or Tukey's multiple comparisons test was used for statistical analyses