Fig. 8
A Representative spots and B quantitative analysis of IFN-γ secreting CD8 + T splenocytes. C In vitro splenocytes proliferation measured by CCK-8 after restimulation by 5 µg mL−1 of HBsAg for 24 or 48 h. D CD69 positive cells among CD19 + B cells, (E) CD4 + T cells, (F) CD8 + T cells, for characterisation of activation measured by flow cytometry. G FasL + cells in CD8 + T cells for characterisation of cytotoxic activity detected by flow cytometry. Data are all expressed as Means ± SD. One-way ANOVA was used for statistical analyse