Table 1 Reported studies on the purification of proteins with biomolecules and functionalized MNPs
From: State of the art on the separation and purification of proteins by magnetic nanoparticles
Composites of core–shell | Size (nm) | Technique of synthesis | Protein target | References |
|---|---|---|---|---|
Covalent immobilization of protein A onto Glu activated Fe3O4@SiO2 MNPs (Fe3O4@SiO2 core–shell MNPs/proteinA) |  ~ 72 | Solvothermal | Anti-EGFR monoclonal antibody (anti-EGFR mAb) | Hou et al. [53] |
GNTA-SPION (3-glycidoxypropyl-trimet hoxy-silane Nα,Nα-bis(carboxymethyl)-l-lysine coupled superparamagnetic iron oxide nanoparticles) | 6.5 | Solvothermal | Recombinant proteins (protein A and α-lysozyme D1.3scFv antibody fragment from Bacillus megaterium cultivation) | Gädke et al. [44] |
SD-SPION (spray-dried GNTA-SPION) | 6.6 | |||
GNTA-SPION (3-glycidoxypropyl-trimet hoxy-silane Nα,Nα-bis(carboxymethyl)-l-lysine coupled superparamagnetic iron oxide nanoparticles) | 8–10 | Non-aqueous sol–gel method | Histidine-tagged protein A (from Bacillus megaterium) | Gädke et al. [43] |
Magnetic particles of azocasein-iron composite (mAzo) | – | Solvothermal | Trypsin (from tilapia fish crude extract) | Alves et al. [1] |
Biofunctional magnetic nanoparticles bound to iminobiotin (Fe3O4/2-iminobiotin) | 30 | Solvothermal | Avidin (from treated egg-white solution) | Sun et al. [104] |
Mesoporous silica coated magnetic microspheres with perfluorooctyl moieties on interior pore walls (Fe3O4@mSiO2-C8F17) | 346 | Surfactant templated sol–gel reaction | Fluorescent-labeled N-linked glycans | Zhao and Deng [141] |
Glucose functionalized magnetic graphene hydrophilic nanocomposite (MagG/Au/Glu) | 15 | Solvothermal | Glycopeptides (from human serum) | Feng et al. [39] |
Biotinylated poly(N-isopropylacrylamide) (pNIPAAm)- modified AuNPs mixed with pNIPAAm-coated iron oxide magnetic nanoparticles (mNPs) (pNIPAAm- AuNPs + pNIPAAm-mNPs) | pNIPAAm- AuNPs: 23 nm | UUsing laboratory syringe pumps | Biomarker protein streptavidin (in spiked human plasma) | Nash et al. [78] |
pNIPAAm-mNPs: 10Â nm | ||||
Bifunctional Au–Fe3O4 Nanoparticles (thiol modified Fe3O4 NPs mixed with Au NPs) | – | – | Arginine kinase (from cell lysate) | Bao et al. [6] |
Magnetic nanoparticles (MNPs) of Fe3O4 immobilized with the ligand-binding domain (LBD) of estrogen receptor alpha (ERα) (LBD-ERα-immobilized MNPs) | 142.4 | Co-precipitation | Phytoestrogens (standard 17β-estradiol (E2)) in plant extracts (Asparagus racemosus) | Busayapongchai and Siri [13] |
Magnetic glyconanoparticles (Supramolecular complexation between Fe3O4@Polyethylene Glycol@Adamantane and β-cyclodextrins conjugated with hepta-mannose (β-CD-(Man)7) and conjugated with poly(mannose) (β-CD-p(Man)280) (Fe3O4@PEG@AD + β-CD (Man)7/β-CD-p(Man)280) | 22 | Thermal decomposition | Concanavalin A (ConA) lectin (from a mixture of ConA and peanut agglutinin (PNA)) | Oz et al. [82] |
Magnetic nanoparticles functionalized with antisense oligonucleotides (Fe3O4@SiO2–COOH NPs) | 14.6 ± 1.1 | – | MicroRNAs (miRNAs) and intracellular proteins | Gessner et al. [46] |
α-Glucosidase functionalized magnetic nanoparticles (αG-MNPs) | 20 | – | α-Glucosidase inhibitors (from Dioscorea opposita Thunb peel extract) | Chen et al. [21] |