State of the art on the separation and purification of proteins by magnetic nanoparticles

Le, Thanh-Do; Suttikhana, Itthanan; Ashaolu, Tolulope Joshua

doi:10.1186/s12951-023-02123-7

Journal of Nanobiotechnology

Table 2 Reported studies on the use of ionic metals functionalized with MNPs for protein purification

From: State of the art on the separation and purification of proteins by magnetic nanoparticles

Technique of synthesis	Size (nm)	Functionalizing metal	Core material	Protein target	References
One-pot hydrothermal	140	Nickel	Iron oxide	Poly-histidine	Liu et al. [72]
–	80	Nickel	Iron oxide	Histidine-tagged proteins	Yao et al. [123]
Ti foils anodization	125–230	Fe₃O₄	TiO₂	Histidine-tagged recombinant proteins	Kupcik et al. [62]
–	15	Nickel	Iron oxide	Enhanced green fluorescent protein	Wang et al. [114]
–	30	lanthanide elements	Iron oxide	Phosphopeptide	Zhai et al. [124]
–	220	Copper	Iron oxide	Bovine haemoglobin	Shi et al. [101]
–	220	Indium oxide	Iron oxide	Phosphopeptide	Jiang et al. [58]
Thermal plasma and chemical method involving co-precipitation of nitrate salts	28–32	Nickel	Iron oxide	Bovine serum albumin	Bhosale et al. [7]
Thermal plasma	240	Nickel–Zinc	Iron oxide	Histidine-tagged proteins	Feczkó et al. [37]
–	6–8	Nickel	Iron oxide	Histidine-tagged proteins	Sahu et al. [96]
Solvothermal Method	200	Nickel	Iron oxide	Histidine-tagged red fluorescent protein	Yang et al. [122]
Coprecipitation method	221	Titanium	Iron oxide	Phosphopeptide	Piovesana et al. [88]
Coprecipitation method	70	Nickel	Iron oxide	Histidine-tagged proteins	Rashid et al. [93]
High-temperature diol reduction method	5	Copper	CoFe₂O₄	Bovine haemoglobin	Li et al. [65]
	–	Nickel	Iron oxide	Pullulanases	Wang et al. [112]
Water-in-oil microemulsion technique	8	Zinc	Iron oxide	Histidine-tagged phycobiliprotein	Chen et al. [22]
–	20	Iron	Iron oxide	Phosvitin phosphopeptides	Zhang et al. [125]
–	10	–	Fe₃O₄–Na₂Ti₃O₇	Albumin and haemoglobin	Zhou et al. [131]
Coprecipitation method	14	Nickel	Cobalt ferrite	Tpv-sHSP 14.3	Aygar et al. [4]
Hydrothermal method	30	Nickel	Iron oxide	Histidine-tagged proteins	Cao et al. [15]
Coprecipitation method	11	Co²⁺	Iron oxide	Benzaldehyde lyase	Tural et al. [108]
–	35	Nickel	Iron oxide	Histidine-tagged proteins	Rashid et al. [93]
Coprecipitation method	30	Nickel	NiFe₂O₄	Histidine-tagged proteins	Mirahmadi-Zare et al. [76]
Water-in-oil microemulsion technique	200	Co²⁺	Iron oxide	Histidine-tagged proteins	Feng et al. [38]
Solvothermal Method	225	Gadolinium	Iron oxide	Phosphopeptides	Jiang et al. [57]
–	36	Zirconium, Nickel and Copper	Iron oxide	Histidine-tagged green fluorescent protein	Fraga García et al. [42]
Coprecipitation Method	75	Nickel	Iron oxide	Histidine-tagged enzyme	Wang et al. [113]
–	9	Nickel	Iron oxide	Histidine-tagged fluorescent DsRed proteins	Bloemen et al. [8]
Coprecipitation Method	75	Nickel	MnFe₂O₄	Histidine-tagged proteins	Rashid et al. [94]
Modified polyol method	68	Nickel	Iron oxide	Single-domain antibody ToxA5.1	Parisien et al. [85]
–	16	Zirconium	Iron oxide	α- and β-caseins	Zhao et al. [130]
–	27–31	Copper	Iron oxide	Bovine haemoglobin and bovine serum albumin	Zhang et al. [129]
One-pot hydrothermal method	83	Nickel	Graphene	Poly-histidine-tagged proteins	Liu et al. [68]
Solvothermal method	281	Copper	CoFe₂O₄	Haemoglobin	Liu et al. [70]

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