State of the art on the separation and purification of proteins by magnetic nanoparticles

Table 3 Synthesized and natural polymers as functionalizing agents of MNPs in protein purification

Method of synthesis	MNP size (nm)	Chelating agent	Core material	Target protein	References
Miniemulsion/solvent evaporation technique	105	Ni²⁺-NTA	Iron oxide	Hexa-histidine-tag green fluorescent protein	Jose et al. [60]
Miniemulsion polymerization	11	P(NIPAM-co-AA)	Magnetic poly(methyl methacrylate)	IgG	Borlido et al. [10]
	215	CMPEI-Ni²⁺	Iron oxide	Histidine-tagged peptides	Chang et al. [19]
Co-precipitation method	50–100	Chitosan	Iron oxide	BSA	Gonzalez et al. [48]
–	98	Polyethyleneimine	Iron oxide	Phosphopeptides	Chen et al. [22]
–	10	NTA-Ni²⁺	Iron oxide	Histidine-tagged peptides	Guo et al. [50]
	10	Carboxymethyl cellulose and chitosan	Iron oxide	Peroxidase enzyme	Kurt et al. [63]
Co-precipitation method	15	Carboxymethyl chitosan	Iron oxide	Lysozyme	Sun et al. [104]
	30	Cibacron Blue F3GA	Iron oxide	BSA	Amiri et al. [2]
	10	Carboxymethyl chitosan	Iron oxide	BSA	Sadeghi et al. [95]
Solvothermal method	20	Polydopamine	Iron oxide	Hydrophobins	Cordova et al. [27]
	–	mPEG	Iron oxide	IgG and BSA	Cheng et al. [23]
Reversible addition − fragmentation chain-transfer polymerization	1000–3000	poly(N-isopropylacrylamide)	Iron oxide	Lactoferrin, BSA, and lysozyme	Paulus et al. [87]
Surfactant free emulsion polymerization	163	N-acetyl-d-galactosamine	poly(HEMA-Man-OPA)	Lectin	Feyzioğlu Demir et al. [40]
Co-precipitation method	200	Chitosan	Iron oxide	IgG	Kavas et al. (2012)
RAFT polymerization	10	10 kDa pNIPAm	Iron oxide	Elastin-like polypeptide receptors	Ta et al. [105]
–	79	Poly(styrene-sulfonate-N-isopropylacrylamide)	Iron oxide	Lysozyme	Zhang et al. [126]

ISSN: 1477-3155