Fig. 5

PEG-CeONPs-carried hUCMSCs resisted ROS-mediated senescence. A Representative colony formation morphology with result quantification (n = 3). B Cell viability was assessed by the CCK-8 assay after treatment with PEG-CeONPs and H₂O₂(n = 3).  C Ki-67 immunofluorescence staining and percentage of positive cells (n = 3). D Representative images of β-galactosidase staining and percentage of senescent cells (n = 3). E WB analysis of P21, P16, IL-1β, IL-6 and TNFα in hUCMSC treated with PEG-CeONPs and H₂O₂ (n = 3). F RT-qPCR analysis of P21, P16, IL-1β, IL-6 and TNFα in hUCMSC treated with PEG-CeONPs and H₂O₂ (n = 3). NS means not significant versus NC, **p < 0.01 versus NC, ^#p < 0.05 versus H₂O₂, ^##p < 0.05 versus H₂O₂