Fig. 3

Nanoparticles physicochemical characterization and in vitro release of 5-FU. A Image of empty F NPs (left) and of 5-FU-loaded F NPs (right) obtained by Transmission Electron Microscopy (TEM). Scale bar: 200 nm. B Stability of empty nanoparticles (NF NPs), functionalized unloaded NPs (empty F NPs) and of 5-FU-loaded F NPs, in cell culture medium at 4 °C for 1 month. C in vitro release profile of 5-FU from F NPs in PBS pH 7.4 at 37 °C during 552h. C. All measurements were done in triplicate and results are presented as mean ± SD