Fig. 4

Gating strategy used to select cells in focus and separate them from the doublets and non-focused events in untreated cells (left image). Gating strategy for single cells from focused cells in the middle. Features for the bivariate dot plot are calculated based on a Brightfield image and using the mask that covers the whole cell (channel 1, ch1). Proof of successful separation of the cells from the non-focused and doublets verified in imaging gallery with respective fluorescence in each channel (Caco-2; SW48; SW480 and HCT-15 cell lines incubated with F NPs along 24 h) (right image)