Fig. 5

Imaging Flow cytometry analysis of cell internalization of F and NF NPs or NF NPs incubated with free anti-CEA (NF NPs + scFv) into A) CEA ^high cell lines (Caco-2 MSS cell line on the left and Sw48 MSI cell line on the right) and into B) CEA-non expressing cell lines (SW480, MSS cell line, on the left and, HCT-15, MSI cell line, on the right). Expression values were normalized to the unstained control. Statistical comparison of functionalized with non-functionalized NPs. *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001