Fig. 6

Evaluation of metabolic activity in CRC MSI and MSS cell lines when incubated with free 5-FU, empty NF NPs, F NPs and 5-FU loaded F NPs at 24 h, 48 h and 72 h. A Metabolic activity assessment of CEA ^high cells. B Cell viability evaluation in CEA ^low cells. C Macrophages metabolic activity. Statistical comparison of 5FU-loaded F NPs with the free drug 5-FU, within each dose range. *p < 0.05, **p < 0.01, ***p < 0.001 or ****p < 0.0001