Fig. 5

ApoV-HS possesses a superior biocompatibility both in vitro and in vivo. a CCK8 assay indicating the proliferation rate of L929 cells (mouse fibroblasts) treated with the leaching solutions from indicated groups. b Fluorescence images and quantification showing the proliferation rate of L929 cells treated with the leaching solutions from indicated groups. Scale bar, 40 μm. Red: Edu staining; Blue: DAPI staining. c Hemolysis rate of apoV-HS and control groups (PBS-HS, Avitene™, Surgifoam®). DI and PBS were set as positive and negative control, respectively. d HE staining showing histological changes of mice skin after subcutaneous implantation with PBS-HS or apoV-HS for 2 and 4 weeks. Mice in control group underwent the same surgical procedures, with no materials implanted. Scale bar, 100 μm. n = 4 per group. e Fluorescence images and quantification showing the CD3⁺ T cells of mice skin after subcutaneous implantation for 4 weeks. Scale bar, 20 μm. Red: CD3 staining; Blue: DAPI staining. ns, no significant