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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells

Fig. 6

BT-EXO-CAP activated the Keap1 / Nrf2 / GPX4 signaling pathway leading to ferroptosis in OS cells. (A) Representative immunoblot of Keap1, Nrf2, and GPX4 protein levels. n = 3. (B) Flow cytometry-based detection of Nrf2-expression. n = 3. (C) Representative immunoblot of Nrf2 protein levels in cytoplasm and nucleus. n = 3. (D) The fluorescence microscope captures the FITC fluorescence of OS cells. (E) Immunoblot analysis of Nrf2 in OS cells. n = 3. (F) Flow cytometry-based detection of FerroOrange and (G) C11-BODIPY fluorescence in OS cells. (H) GSH levels in OS cells. n = 6. * p < 0.05, *** p < 0.001. Scale bar: D 100 μm

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Journal of Nanobiotechnology

ISSN: 1477-3155

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